UCLA researchers have developed a DNA sequencing method that makes use of small format (8cm x 11cm) gel electrophoresis systems with virtually no loss of performance characteristics. Primary benefits of this system are in savings of time and labor. For example, estimates are that the time from beginning a sequencing experiment to having DNA sequence data entered on a computer can be reduced from a minimum of 16-24 hours to less than 6 hours. Small format gels are especially useful in reducing time and labor required in the preparation of plates and the pouring of gels.

This advance is made possible, in part, by the rapid development and affordability of computer-assisted scanning equipment. Discrimination between incrementally-sized DNA fragments is somewhat more difficult using small format gels, but the fidelity of even this small gel matrix is such that, with magnification, automated gel scanners are able to read with high accuracy to lengths greater than 300bp.

A significant percentage of DNA sequencing activity as currently practiced in research laboratories could be carried out using small format gel systems.

Reference: UCLA Case No. 1993-561

US Patent Number: 5,800,993