BACKGROUND:  Monoclonal antibodies (mAb) are important research tools in biochemistry, molecular biology and medicine. They can be made to bind specifically to any given substrate, and are also used to detect and purify substances. Monoclonal antibodies are intended to be used for applications including immunoblotting (western), immunoprecipitation (IP), and immunohistochemistry (IHC). Research areas, such as stem cell research and cancer biology, can benefit from the development of monoclonal antibodies. Currently, there are only polyclonal antibodies available for research use in these areas. There is a need for a high quality monoclonal antibody to bring value to the research community.

INNOVATION:  UCLA researchers have developed a monoclonal antibody for CDK2AP-1. CDK2AP-1 is an essential gene for early embryonic development, and also a cell cycle regulator by the inhibition of G1/S transition through downregulation of CDK2. Investigators have determined that CDK2AP-1 confers competency for the proper differentiation of murine ES cells. Therefore, the CDK2AP-1 mAb will be useful in the immuno-detection of the stem cell differentiation competency factor CDK2AP-1.

POTENTIAL APPLICATIONS 

• Research reagent/tool used for applications that include immunoblotting (western), immunoprecipitation (IP), and immunohistochemistry (IHC)
• Immuno-detection of the cell cycle regulator and stem cell differentiation competency factor CDK2AP-1

ADVANTAGES

• There is not currently a monoclonal antibody for CDK2AP-1 in the research community
• High quality reagent and renewable resource
• Single band western detection and robust for immunoprecipitation
• Performance of monoclonal antibodies far exceeds that of polyclonal antibodies

DEVELOPMENT-TO-DATE:  Investigators have produced the CDK2AP-1 monoclonal antibody reagent. As well, investigators have created CDK2AP-1 knockout mice in their research regarding early embryonic development. A conditional knockout mouse is also close to being developed.

Related Papers (Selected)

• Tsuji T, Duh F-M, Latif FL, Popescu NC, Zimonjic DB, McBride J, Matsuo KM, Ohyama H, Todd RT, Nagata EN, Terakado N, Sasaki A, Matsumura T, Lerman MI, Wong DTW. Cloning, Mapping, Expression, Function and Mutation Analyses of the Human Ortholog of the Hamster Putative Tumor Suppressor Gene doc-1. J Biol Chem 1998; 273: 6704-6709. [more]
• Shintani S, Ohyama H, Zhang X, McBride J, Matsuo K, Tsuji T, Todd R, Lerman M, Wong DTW. p12DOC-1 is a novel CDK2-associated protein. Mol Cell Biol 2000; 20:6300-6307. [more]
• " Kim, Y, Ohyama, H, Patel, V, Figueiredo, M L, and Wong, D T. Mutation of Cys10 105 inhibits dimerization of p12CDK2-AP1 and its growth suppressor effect. J Biol Chem 2005; 280: 23273-23279. [more]

Reference: UCLA Case No. 2008-679