INNOVATION:  UCLA researchers have developed a genetically altered transposase that, when used with an optimized buffer, is found to have a 280-fold increased transposition activity compared to the wild type transposase and original buffer. This system decreases the site preference of the transposition event, resulting in a more even distribution throughout the target gene. This technology provides a useful tool for improved transposon efficiency.

POTENTIAL APPLICATIONS 

• Improved Transposon efficiency for insertional mutagenesis and DNA sequencing applications

ADVANTAGES

• The transposon system has 280-fold greater activity compared to conventional systems
• This technology results in a decreased site preference of the integration event

Reference: UCLA Case No. 2008-381